No sialylation was keyed. N-glycans with non-bisecting GlcNAc (9, 10 %), non-fucosylation (10, 13 %), non-α-galactosylation (26, 46 %), and non-β-galactosylation (6, 8 %) were also described. The activity (100 %) of biALP was reduced to 37 ± 0 % (by de-fucosylation), 32 ± 2 % (by de-α-galactosylation), and 0 ± 0 % (by de-β-galactosylation), comparable to inhibition by 10(-4)