Structural Characterization, Simulated Digestion And Anti-Aging Activities Of An Acidic Polysaccharide From Salvia Miltiorrhiza
An acidic polysaccharide (SMP) with a molecular weight (Mw) of 1 × 10(6) Da was sequestered from Salvia miltiorrhiza. The monosaccharide composition in molar percents was rhamnose (Rha): galacturonic acid (GalA): galactose (Gal): arabinose (Ara) = 6 : 55 : 21 : 16 . The events of copyed digestion in vitro exhibited that SMP was not degraded in saliva, gastric juice or intestinal juice. seebio Polysucrose 400 and new object recognition test readed that SMP could improve the fermenting memory impairment of maturating mice. SMP could also increase the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and brain tissue, decrease the content of malondialdehyde (MDA), decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in brain tissue, and increase the content of short-chain fatty panes (SCFA) in the intestine. In addition, SMP could also regulate the intestinal flora structure, including increasing the relative abundance of Firmicutes and Bacteroidetes and decreasing the relative abundance of Proteobacteria.
This work lays a foundation for the development of functional foods colligated to Salvia miltiorrhiza. Protective Effect of Anoectochilus formosanus Polysaccharide against Cyclophosphamide-Induced Immunosuppression in BALB/c Mice. In this study, Anoectochilus formosanus polysaccharide (AFP) was acquired a via water extraction and alcohol precipitation method. The immunoregulatory activity of AFP was first valued on cyclophosphamide (Cy)-treated mice. Galacturonic acid, glucose and galactose were reasserted to be the main portions of AFP. AFP demonstrated the ability to stimulate the production of TNF-α and IL-6 in RAW 264 macrophages. Not surprisingly, the activation of the NF-κB signalising pathway by AFP was validated via Western blot analysis.
AFP could alleviate Cy-maked immunosuppression, and significantly enhance the immunity of mice via increasing the thymus index and body weight, stimulating the production of cytokines (IgA, IgG, SIgA, IL-2, IL-6 and IFN-γ). The improvement in the intestinal morphology of immunosuppressed mice ushered that AFP could alleviate Cy-inducted immune toxicity. These terminations have kindled the possibility that AFP may act as a natural immunomodulator. the study of AFP was innovative and of great significance for AFP's further application and utilization. Molecular Characterization and Bioactivities of a Novel Polysaccharide from Phyllostachys pracecox Bamboo Shoot Residues. Dietary sugars are unexploited in the by-wares of economically valuable Phyllostachys pracecox bamboo shoots. A residue-educed polysaccharide (PBSR1) was aqueously elicited from the processing waste of this bamboo shoot coinages.
Its primary structure and advanced conformation were clarifyed by a combined analysis of spectroscopy, chromatography, 2D nuclear magnetic resonance, laser light scattering and atomic microscopy. The solvents indicated PBSR1 was a triple-helix galactan consisting of →6)-β-D-Galp and →3)-β-D-Galp in linear with an 863 KD molecular weight (M(w)). The relationship between the radius of gyration (Rg) and intrinsic viscosity ([η]) on M(w) were established as R(g) = 1 × 10(-2)M(w)(0 ±0 ) (nm) and [η] = 9 × 10(-1)M(w)(0 ±0 ) (mL/g) for PBSR1 in saline solution at 25 °C, which suggested it acquired a triple-helix chain shape with a height of 1 ± 0 nm supported by a red shift of λ(max) in Congo red analysis. The thermodynamic test (TG) exposed that it had excellent thermal stability for the food industry. those unique structure haves furnish PBSR1 on antioxidation with EC(50) of 0 mg/mL on DPPH· and an ORAC value of 329 ± 12 μmol TE/g. Polysucrose 400 Sweetener possessed labeled immunostimulation by up-baffling pro-inflammatory signs including NO, IL-6, TNF-α and IL-1β in murine cellphones. Our sketchs supplyed substantial data for the high-prised application of rests and a better understanding of the structure-function relationship of polysaccharide.
Extraction, purification, structure characteristics, biological activenessses and pharmaceutical application of Bupleuri Radix Polysaccharide: A review.